<!DOCTYPE article PUBLIC "-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" "journalpublishing.dtd">
<article article-type="brief-report" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML">
<front>
<journal-meta>
<journal-id journal-id-type="nlm-ta">Our Dermatol Online</journal-id>
<journal-title>Our Dermatol Online</journal-title>
<issn pub-type="epub">2081-9390</issn>
<publisher>
<publisher-name>Our Dermatology Online</publisher-name>
<publisher-loc>Poland</publisher-loc>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="publisher-id">OURD-9-22</article-id>
<article-id pub-id-type="doi">10.7241/ourd.20181.6</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Brief Report</subject>
</subj-group>
</article-categories>
<title-group>
<article-title>Kefir and champagne vinegar to defeat bacterial vaginosis in woman, avoiding oral metronidazole, clindamycin and bothersome douchings</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname>Brzezinski</surname>
<given-names>Piotr</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="aff" rid="aff2">2</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Martin</surname>
<given-names>Lorenzo</given-names>
</name>
<xref ref-type="aff" rid="aff3">3</xref>
<xref ref-type="corresp" rid="cor1"/>
</contrib>
</contrib-group>
<aff id="aff1"><label>1</label><italic>Institute of Biology and Environmental Protection, Pomeranian Academy, ul. Arciszewskiego 22A, 76-200 Slupsk, Poland</italic></aff>
<aff id="aff2"><label>2</label><italic>Department of Dermatology, 6<sup>th</sup> Military Support Unit, Ustka, Poland</italic></aff>
<aff id="aff3"><label>3</label><italic>Department of Pharmaceutical Biotechmologies, University of Siena, Siena, Italy</italic></aff>
<author-notes>
<corresp id="cor1">
<bold>Corresponding author:</bold> Lorenzo Martini, E-mail: <email xlink:href="martinil163@libero.it">martinil163@libero.it</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub">
<year>2018</year>
</pub-date>
<volume>9</volume>
<issue>1</issue>
<fpage>22</fpage>
<lpage>24</lpage>
<history>
<date date-type="received"><day>09</day><month>06</month><year>2017</year></date>
<date date-type="accepted"><day>12</day><month>09</month><year>2017</year></date>
</history>
<permissions>
<copyright-statement>Copyright: &#x000a9; Our Dermatol Online 1</copyright-statement>
<copyright-year>2018</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0">
<p>This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p>
</license>
</permissions>
<abstract>
<p>Scope ouf our study is to treat with natural remedies vaginitis in woman, when it has been detected the disease originates from bacterical assault (<italic>Gardnerella vaginalis</italic> and/or <italic>Streptococca spp</italic>.) in order to avoid the administration of perilous antibiotics and elicit sexual desire and eliminate pain during urination in the woman who has suffered from this disease after 4-5 days only. We have to proceed with the preliminary phase of a simplest test (the ammin whiff test) and determine the type of vaginitis and thus treat it using champagne or cider vinegar to adjust mucosal pH and kefir, a fermented beverage, that is extremely rich in mesophyllic bacteria, apt to reveal an important and suggestive function regard vaginal microbes.</p>
</abstract>
<kwd-group>
<kwd>Vaginitis</kwd>
<kwd>Vulva</kwd>
<kwd>Kefir</kwd>
<kwd>Gardnerella vaginalis</kwd>
<kwd>Champagne vinegar</kwd>
</kwd-group>
</article-meta>
</front>
<body>
<sec id="sec1-1" sec-type="intro">
<title>INTRODUCTION</title>
<p>Vaginitis is an inflammation of the vagina and possible vulva It can result in discharge, itching and pain, and is often associated with an irritation or infection of the vulva. Infected women may also be asymptomatic.</p>
<p>It is usually due to infection. The three main kinds of vaginitis are bacterial vaginosis (BV), vaginal candidiasis, and trichomoniasis. A woman may have a combination of vaginal infections at one time.</p>
<p>Bacterial vaginosis is generally sustained by gardnerella vaginalis and it is preferable to individuate if the woman who suffers from vaginitis, has this infection instead of fungal assaults, in order to treat the disease by the best and more rapid way and to avoid to administer to her antimycotics instead of antibiotics and vice versa and moreover, to trace the best path to cure the malaise owing to natural medicaments.</p>
<p>Gardnerella is treated commonly by metronidazole [<xref ref-type="bibr" rid="ref1">1</xref>] clindamycin [<xref ref-type="bibr" rid="ref2">2</xref>-<xref ref-type="bibr" rid="ref4">4</xref>].</p>
<p>Now it is well acquainted that amongst the manifold side effects given by metronidazole, the most common are numbered in <xref ref-type="fig" rid="F1">Box 1</xref>.</p>
<fig id="F1">
<label>Box 1</label>
<caption>
<p>The side effects by metronidazole</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="OURD-9-22-g001.tif"/>
</fig>
<p>severe skin reaction -- fever, sore throat, swelling in your face or tongue, burning in eyes, skin pain, followed by a red or purple skin rash that spreads (especially in the face or upper body) and causes blistering and peeling.</p>
<p>And amongst the side effects given by assumption of clindamycin, these are the most severe (<xref ref-type="fig" rid="F2">Box 2</xref>).</p>
<fig id="F2">
<label>Box 2</label>
<caption>
<p>The side effects by clindamycin</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="OURD-9-22-g002.tif"/>
</fig>
<p>A recent paper [<xref ref-type="bibr" rid="ref5">5</xref>] asserts that in case of aggression of gardnerella or other microbiota typical of vaginosis, the physiological production of lactic acid and other short chain fatty acids, apt to mantain pH 4,5 (optimal for combating the occurrence of yeasts or bacterial infections in vagina and vulva), decreases abruptely or goes to end.</p>
<p>These bacteria are able, indded, to produce long chain fatty acids, as acetates, propionates, butyrates, and succinates.</p>
<p>A simplest method to distinguish if the vaginitis is originated by bacterial aggressions or fungal assaults is the ammin whiff test, that is used to determine the quantity of gardnerella vaginalis in order to adjust the dosage of antibiotics, and in the preliminary phase, during the preparation of the kit, and using a 10&#x0025; KOH solution, a strong fragrance of fish comes out and this is the indisputable sign of presence of these bacteria and/or <italic>Streptococca spp</italic> and the presence or co-presence of fungi or Trichomoniasis is exorcised at all.</p>
<p>Other discovered too that mesophyllic bacteria are able to oxidate octanoates at temperatures comprised between 7.5&#x00B0;C and 40&#x00B0;C, this fact signifies that mesophyllic bacteria are capable to denature vaginal commensal bacteria [<xref ref-type="bibr" rid="ref6">6</xref>].</p>
<p>Kefir is a fermented milk drink that originated in the north Caucasus Mountains made with kefir &#x201C;grains&#x201D;, a yeast/bacterial fermentation starter. It is prepared by inoculating cow, goat, or sheep milk with kefir grains [<xref ref-type="bibr" rid="ref7">7</xref>] and moreover these grains called kefiran contain bacterial colonies especially mesophyllic living symbiotically with yeasts.</p>
<p>An acidic milieu (below 4.5) must be reached and naturally speaking the best way is to cleanse vagina with douche containing pure cider vinegar, or to insert a cotton ball soaked in cider or champagne vinegar and let it stay all through the night inside vagina.</p>
<p>Kefir must be spread as a liquid emulsion every time the woman has urinated and washed with lukewarm water and savon de marseille (that shows a mild antibacterial function too).</p>
<p>Kefir must be kept in fridge, for this, the woman had to keep the beverage in fidge at home (or even at work).</p>
<p>Applications were 6-7 pro day.</p>
</sec>
<sec id="sec1-2" sec-type="materials|methods">
<title>MATERIALS AND METHODS</title>
<p>We have recruited a woman (27 y. old) who had declared to feel a severe annoyance during urinating, perpetual itching during day and night in her vagina and vulva.</p>
<p>She was very embarassed when she was at work or spent her time in social occasions.</p>
<p>She was sure not to suffer or have suffered from Candida albicans since the Candida anti-body blood test was negative, she has always avoided high sugar diet, never taken antibiotics, did not suffer from chronic stress, did not assume contraceptive pill, and did not have diabetes.</p>
<p>She used to wear banana hammock, even she used to wash it almost three times pro week. She has thought she had mistaken the usage of the detergent for washing her intime underwear.</p>
<p>First of all, we have effectuated the ammine whiff test (only the preliminary phase) and we assayed the penetrating fish odour pouring a KOH 10&#x0025; solution directly in her discharge, but we identified by electronic microscope even the presence of Gardnerella and Streptococca spp.</p>
<p>We began the experimentations following this schedule:</p>
<p>the volunteer had to insert a cotton ball (as it were a normal vaginal swab) all the night long for five nights, almost 7-8 hours.</p>
<p>During the day, she had to spread kefir (kept in cool) every time she had urinated.</p>
</sec>
<sec id="sec1-3" sec-type="results">
<title>RESULTS</title>
<p>From <xref ref-type="table" rid="T1">Table 1</xref> it is possible to observe that after the second day discharge and unpleasant odour (simply adding KOH solution to her urine at the mornig) disappeared at all.</p>
<table-wrap id="T1">
<label>Table 1</label>
<caption>
<p>Responses of ammin whiff tests effectuated every day for five days</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="OURD-9-22-g003.tif"/>
</table-wrap>
<p>From <xref ref-type="table" rid="T2">Table 2</xref> it is possible to behold the feeling of itching, according to a conventional scale:</p>
<p><list list-type="simple">
<list-item>
<p>a) Itching during day and night</p>
</list-item>
<list-item>
<p>b) Itching only during the day</p>
</list-item>
<list-item>
<p>c) Itching only after urinated</p>
</list-item>
<list-item>
<p>d) Mild itching through all the day, but tolerable</p>
</list-item>
<list-item>
<p>e) Absence of itching</p>
</list-item>
</list>
</p>
<table-wrap id="T2">
<label>Table 2</label>
<caption>
<p>Sensation of itching during the treatment of five days.</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="OURD-9-22-g004.tif"/>
</table-wrap>
</sec>
<sec id="sec1-4" sec-type="discussions|conclusions">
<title>DISCUSSIONS AND CONCLUSIONS</title>
<p>It is suggestive to herald that avoiding drastic antibiotics, endowed of side effects too often risky, is possible to treat bacterial vaginosis (when the co-presence or co-presence of fungi are exorcised), a woman suffering from bacterial vaginosis, who declares pain in urinating, lack of sexual desire, extreme itching and malodorous abrupt discharges, may be treated by simplest natural remedies as champagne or cider vinegar (to be applied as a swab during the night) and spreading kefir 6-7-8 times pro day.</p>
</sec>
</body>
<back>
<ref-list>
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<fn-group>
<fn fn-type="supported-by">
<p><bold>Source of Support:</bold> Nil</p>
</fn>
<fn fn-type="conflict">
<p><bold>Conflict of Interest:</bold> None declared.</p>
</fn>
</fn-group>
</back>
</article>