EPONYMS IN THE DERMATOLOGY LITERATURE LINKED TO STAINS USED IN SKIN BIOPSIES

Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes [1]. Hematoxylin and eosin stain (H&E stain or HE stain), is the routine staining for skin biopsies. However, in some circumstances, dermatopathologists use other stains. For example, the Fontana-Masson Stain is used for melanin as it can be difficult to decide whether a brown pigment is lipofuscin, hemosiderin, or melanin. At pH4, melanin granules reduce silver nitrate to metallic silver, a histochemical reaction that reveals accumulations of black material wherever melanin is located. The Fontana-Masson Stain can be used, also, to detect melanin of dematiaceous fungi [2]. Each stain may give a particular colour to a specific structure in the tissue. For example, Alizarin red binds directly to calcium ions, resulting in an orange-red color [3]. In Foote’s or Snook’s stain, the reticulin fibers will be black. Leder stain (Naphthol ASD chloroacetate esterase), will stain Mast cell cytoplasm red (not dependent on presence of granules). Also stains myloid cells (example, leukemia cutis). Lipid will be stained by Oil red O (requires fresh frozen tissue, as lipid is removed during processing). In addition to Sudan black and Osmium tetroxide (both require fresh tissue). Iron will be stained by Prussian blue, also known as Perls stain, after its inventor, German pathologist Max Perls (1843-1881). In this stain ferric ions react to form a deep blue. It is useful to distinguish melanin from hemosiderin (example:hemosiderin in pigmented purpuric dermatosis). It does not demonstrate iron in intact red blood cells. There are also several other stains which are not used commonly in dermatopathology. For example: Movat’s stain which is a pentachrome stain originally developed by Henry Zoltan Movat in 1955 and later modified by H. K. Russell, Jr. in 1972. It is used to highlight the various constituents of connective tissue. In this stain, the nuclei , elastic fibres will be black; collagen fibres, reticular fibres will be yellow; ground substance, mucin will be blue; Fibrin will be bright red Muscle will be red. Also, Weigert’s elastic stain which will show elastic fibers, blue coloured while cell nuclei gets red or blue. It is named for, Karl Weigert, or Carl Weigert (1845-1904), (Fig. 1), who was a German pathologist. There are several eponyms linked to the stains used in dermatopathology practice. In Table I [4-19], we listed some examples. EPONYMS IN THE DERMATOLOGY LITERATURE LINKED TO STAINS USED IN SKIN BIOPSIES

Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image.Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes [1].Hematoxylin and eosin stain (H&E stain or HE stain), is the routine staining for skin biopsies.However, in some circumstances, dermatopathologists use other stains.For example, the Fontana-Masson Stain is used for melanin as it can be difficult to decide whether a brown pigment is lipofuscin, hemosiderin, or melanin.At pH4, melanin granules reduce silver nitrate to metallic silver, a histochemical reaction that reveals accumulations of black material wherever melanin is located.The Fontana-Masson Stain can be used, also, to detect melanin of dematiaceous fungi [2].Each stain may give a particular colour to a specific structure in the tissue.For example, Alizarin red binds directly to calcium ions, resulting in an orange-red color [3].In Foote's or Snook's stain, the reticulin fibers will be black.Leder stain (Naphthol ASD chloroacetate esterase), will stain Mast cell cytoplasm red (not dependent on presence of granules).Also stains myloid cells (example, leukemia cutis).Lipid will be stained by Oil red O (requires fresh frozen tissue, as lipid is removed during processing).In addition to Sudan black and Osmium tetroxide (both require fresh tissue).Iron will be stained by Prussian blue, also known as Perls stain, after its inventor, German pathologist Max Perls (1843-1881).In this stain ferric ions react to form a deep blue.It is useful to distinguish melanin from hemosiderin (example:hemosiderin in pigmented purpuric dermatosis).It does not demonstrate iron in intact red blood cells.There are also several other stains which are not used commonly in dermatopathology.For example: Movat's stain which is a pentachrome stain originally developed by Henry Zoltan Movat in 1955 and later modified by H. K. Russell, Jr. in 1972.It is used to highlight the various constituents of connective tissue.In this stain, the nuclei , elastic fibres will be black; collagen fibres, reticular fibres will be yellow; ground substance, mucin will be blue; Fibrin will be bright red Muscle will be red.Also, Weigert's elastic stain which will show elastic fibers, blue coloured while cell nuclei gets red or blue.It is named for, Karl Weigert, or Carl Weigert (1845-1904), (Fig. 1), who was a German pathologist.There are several eponyms linked to the stains used in dermatopathology practice.In Table I [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19], we listed some examples.

Remarks
Bodian stain [4] Nerve fibers will appear black with this stain.Nerve fiber can also be stained by PGP 9.5 and neurofilament.Named after David Bodian (1910-1992), (Fig. 2).Bodian received his Ph.D. in anatomy in 1934 and his M.D. in 1937 from the University of Chicago.He made major contributions to the knowledge of the basic structure of nerve cells.
Table I.Selected Eponyms in the dermatology literature linked to stains used in skin biopsies.
© Our Dermatol Online 4.2013 569 Eponyms in the dermatology literature linked to stains used in skin biopsies

Remarks
Fite stain [6] Also known as, Fite-Faraco stain.Fite is preferred for "partially acid-fast'' organisms such as lepra bacilli, atypical mycobacteria, and Nocardia.Fite preseves color due to use of peanut oil before staining and gentle decolorization.George Liddle Fite (1933-1993), (Fig. 4), who was arguably the most important American figure in the fight against leprosy.The crowning achievement of a life devoted to the treatment of leprsosy was a chief pathologist of the laboratory at the States Leprosarium in Carville, Louisiana.
Giemsa stain [7] It stains myeloid and mast cell granules purple (it is the heparin in the mast cells that is staining).Also good for many types of organisms, including bacteria, Leishmania, and Histoplasma.Gustav Giemsa (1867-1948), (Fig. 5), was a German chemist and bacteriologist who was a native of Medar-Blechhammer.Giemsa improved the Romanowsky stain (Eosin Yand Methylene Blue) by stabilizing this dye solution with glycerol.This allowed for reproducible staining of cells for microscopy purposes.Romanowsky staining was named after the Russian Physician, Dmitri Leonidovich Romanowsky (1861-1921), who invented it in 1891.
Gomori's aldehyde-fuchsin stain [8,9] Stain elastic tissue purple.Elastic tissue can be also stained by Verhoeff von Gieson (blue-black), and by Orcein-Giemsa (black).It is named for György Gömöri also George Gömöri or George Gomori or George Gömöri , who was a Hungarian-American physician who became famous as histochemist.
Gram staining [10] Gram staining (or Gram's method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative).Named for Hans Christian Gram (1853-1938), (Fig. 6), who was a Danish bacteriologist who developed the technique while working with Carl Friedländer in the morgue of the city hospital in Berlin in 1884.Brown-Hopps tissue Gram stain is a modification of Brown-Brenn technique.Gram-positive organisms stain blue and Gramnegative organisms stain red.
Masson's trichrome stain [11] In this stain, Collagen will appear blue\green and muscle,nerve, and keratin will be red.It is a three-colour staining protocol used in histology.The recipes evolved from Claude L. Pierre Masson's, original formulation to different specific applications, but all are suited for distinguishing cells from surrounding connective tissue.

Von Kossa Stain [16]
A silver stain that stains calcium salts black originally developed by Von Kossa in 1901.
It is one of the Silver stains, which include, Dieterle stain and Steiner stain (a modified Dieterle stain).It stains spirochetes black.
Ziehl-Neelsen stain [18,19] Also known as the acid-fast stain, which is used to identify acid-fast bacteria.In this stain, Mycobacteria will appear bright red.Dr. Franz Ziehl (1857-1926), (Fig. 13), was a German bacteriologist.He was a professor in Lübeck.
Table I.Selected Eponyms in the dermatology literature linked to stains used in skin biopsies (continued).
He was the chair of anatomic pathology at the hospital and medical school at Strasbourg, France.In 1927, while he was 46 years old, Masson resigned his position at Strasbourg and a ccepted the position of chief of anatomic pathology at the University of Montreal Medical School.Pierre Masson died at the age of 79 years.He is buried, as he wished, at the cemetery of Notre-Dame-des-Neiges, atop Mont Royal, where today one has a grand view of the University of Montreal.Table I.Selected Eponyms in the dermatology literature linked to stains used in skin biopsies (continued).