Ass. Prof. Katarzyna Borowska – Poland
Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin, 11 Radziwiłowska Street, 20-080 Lublin, Poland
tel. (017) 866 12 55
e-mail: k_borowska@wp.pl
1995-2001 Medical Univesity of Lublin Faculty of Medicine the final grade: very good.
04.2002 The defense of the graduation thesis: „The strategic analysis of hypothetical dermatological centre” Warsaw School of Economics Post-graduate studies in Health Care Centres Management the final grade: very good Supervisor: Prof. dr hab. Piotr Jeżowski
28.05.2007 The defense of the doctoral thesis with honours „The comparative evaluation of the narrow and wide range of UVB radiation on the histomorphologic and ultrastructural picture and the apoptosis process in experimental animals' skins”. Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin Supervisor: Prof. dr hab. n. med. Krystyna Czerny
28.04.2008 State specialization examination Dermatology and Venereology Department of Dermatology Military Institute of Medicine, Warsaw, Specialization supervisor: Prof. zw. dr hab. n. med. Stanisław Zabielski
2001-2002 Autonomus Public Clinical Hospital no.4 of Medical University of Lublin Postgraduate internship
2002 Department of Dermatology Military Institute of Medicine, Warsaw Postgraduate internship
2005-2007 M.D. (Doctor of Medicine) candidate in Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin
2003-2008 Assistant in Department of Dermatology Military Institute of Health, Warsaw
2008 Adjunct in Department of Cosmetology, University of Information Technology and Managment in Rzeszow.
14.11.2013 „The study of transdermal delivery of 8-methoxypsoralene with the PAMAM dendrimers in the local PUVA method”, which documents publications within the monothematic cycle published after M.D. Diploma:
1. PAMAM dendrimers as solubilizers and hosts for 8-methoxypsoralene enabling transdermal diffusion of the guest. International Journal of Pharmaceutics 398 (2010) 185–189.
2. Effect of polyamidoamine dendrimer G3 and G4 on skin permeation of 8 methoxypsoralene—In vivo study. International Journal of Pharmaceutics 426 (2012) 280– 283.
3. Transdermal delivery of 8-methoxypsoralene mediated by polyamidoamine dendrimer G2.5 and G3.5-In vitro and in vivo study. International Journal of Pharmaceutics. 436 (2012) 764– 770.
I was honoured to be the author of the studies conceptions and the main executor in all of the publications that were significant for the academic achievements on the topic of „The study of transdermal delivery of 8-methoxypsoralene with the PAMAM dendrimers in the local PUVA method” . The work was presented on the conference in India:
Borowska K., Głowniak K., Sieniawska E., Radej S. Transdermal delivery of 8-methoxypsoralene mediated by polyamidoamine dendrimer. 7th International Society for the Development of Natural Products (ISDNP) Symposium, India, Amity Institute for Herbal and Biotech Products Development.
The academic purpose discussion of the above works and their results as well as the discussion of their implication. The purpose of orginal studies published in International Journal of Pharmaceutics were the improvement transdermal delivery of 8-metoxypsoralen (8-MOP) in local PUVA mediated by polyamidoamine dendrimer (PAMAM). The first step was syntheses of PAMAM dendrimers of full-generation G3, G4 and half-generation G2.5, G3.5. The purpose of this study was to investigate the efficacy of PAMAM dendrimers conjugated with 8-MOP for delivery of 8-MOP in vitro study through polivinyldifluoride membrane (PVDE) and prepared pig ear skin (PES) using Franz diffusion cell and in vivo study through the skin of experimental animals (hairless rat skin). The tissue concentration of 8-MOP in hairless rat skin were analyzed by High Performance Liquid Chromatography (HPLC). Detailed distribution of 8-MOP in skin layers and cellular structures were analyzed using laser scanning microscopy (CLSM). 7-Aminoactinomycin D (7-ADD, Invitrogen USA) possesses strong affinity to double-stranded DNA and was used as a fluorescent marker for DNA for identification of cellular nuclei. In vitro and in vivo studies showed that half-generation G2.5 and G3.5 PAMAM dendrimers are able to facilitate transdermal delivery of 8-MOP in vitro (Franz diffusion cell) and in vivo (in local PUVA therapy). Half-generation dendrimers (G2.5 and G3.5 PAMAM) are effective enhancers of transdermal delivery of 8-MOP in relation to full generation dendrimers (G3 and G4 PAMAM). The necessary dose of ultraviolet A irradiation and effectiveness of PUVA therapy depends on 8-MOP concentration in the skin. Higher concentration of 8-MOP delivered to the skin allows to reduce UVA radiation dosage and potential side effects for example photocarcinogenesis. Tt seems that higher 8-MOP concentration achieved by PAMAM carriers will allow to reduce patients UVA exposure and thus could contribute to improved effectiveness and safety of PUVA therapy. Potentially, local PUVA together with PAMAM can become an alternative for systemic PUVA for patients with contraindications for oral 8-MOP. The first study was histological experiment about the infuence of ethanol and progesteron on placenta rats in Department of Histology and Embriology of the Medical University in Lublin. Head of department was Prof. dr n. med. Krystyny Czerny. Scientific supervisor was Prof. dr n. med. Barbary Jodłowskiej-Jędrych. This experiment shows the morphological changes in placenta rats. Comperative observations showed atrophic cells of cytotrophoblast, syncytiotrofoblast and Houbauer cells. The next study was experiment shows changes in liver sinusoides after exposure to ethanol and progesteron in rats. On the basis of the above material, I participated in authoring several academic presentations and articles.
Śledz M., Borowska K., Biss M., Tokarczyk R. The influence of ethanol and progestogen on placenta in rats. 9th International Scientific Students' Conference for Students and Young Doctors. Gdańsk, 10-12 May 2001. Abstr. book, s. 146.
Tokarczyk R., Biss M., Śledz M., Borowska K. The influence of ethanol and progestogen on hepatocytes' structure in pregnant rats. 9th International Scientific Students' Conference for Students and Young Doctors. Gdańsk, 10-12 May 2001. Abstr. book, s. 147.
Śledz M., Borowska K., Biss M., Tokarczyk R. The influence of ethanol and progesteron on placenta in rats. International Congress of Medical Students and Young Physicians. Poznań, 20-22 May 2001. Abstr. book, s. 7-8.
Śledz M., Biss M., Borowska K., Tokarczyk R. The influence of ethanol and progestogen on placenta in rats. 39th Symposium of the Medical Students' Research Association Medical University of Lublin. Lublin, 1 December 2001, Abstr. book .46.
Śledz M., Biss M., Borowska K., Tokarczyk R. Etanol, a łożysko u szczura białego. XXXIV Ogólnopolska Sesja Naukowa Studenckiego Towarzystwa Naukowego Pomorskiej Akademii Medycznej. Szczecin, 5-6 December 2001. Abstr. book. 65
Śledz M., Borowska K., Biss M., Tokarczyk R. The nuclei of rats placenta after exposure to ethanol and progestogen. Przeglad Lekarski 2002 t. 59 supl. 2, s. 49. Śledz M., Borowska K., Biss M., Tokarczyk R. Changes in liver sinusoides after exposure to ethanol and progestogen in rats. Przeglad Lekarski 2002 t. 59 supl. 2, s. 47.
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